Table 2.

List of stem cell walls proteins which are differentially regulated in tomato genotypes WVa700 (susceptible) after R. solanacearum challenge. The listed proteins are those consistently reproduced in three biological replications and are statistically significant (Student’s t test, P ≤ 0.05)

Spota	Identityb	Accessionc	Organismd	Scoree	Mr/pIf	Peptidesg	Coverageh	Regulationi	SiP-SePj
1	α-galactosidase, putative	Q9FWV8	Oryza sativa	137	44.66/5.47	2	5.6	0.58	Y
2	Disulfide isomerase like protein	Q38JJ2	Solanum tuberosum	153	39.49/5.62	2	8.1	0.61	Y
3*	Peroxidase	Q07446	Solanum lycopersicum	273	35.99/7.52	5	17	2.99	Y
4	Peroxidase cevi16	Q4A3Y6	Solanum lycopersicum	170	31.74/7.71	3	15	1.86	0.44
5	Xyloglucan endotransglucosylase-hydrolase XTH7	Q6RHX8	Solanum lycopersicum	184	33.46/7.57	2	11	0.59	Y
6	Basic 30 kDa endochitinase	Q05538	Solanum lycopersicum	266	34.34/6.19	4	18	3.56	Y
7	Triose phosphate isomerase	Q6T379	Solanum chacoense	411	27.04/5.73	8	36	1.3	0.67
8	PR 5-like protein	Q7Y1P9	Solanum lycopersicum	144	27.52/5.76	2	14	2.88	Y
9	Translation initiation factor 5A-4	Q9AXQ3	Solanum lycopersicum	255	17.51/5.60	3	28	0.3	0.23
10	Translation initiation factor 5A-4	Q9AXQ3	Solanum lycopersicum	255	17.51/5.60	3	28	0.37	0.23
11	Translation initiation factor 5A-1	Q9AXQ6	Solanum lycopersicum	139	17.30/5.71	2	18	0.66	0.23
12	Glycine-rich protein	Q04130	Solanum lycopersicum	272	73.31/9.98	4	43	0.52	0.47
13	Glycine-rich protein	Q04130	Solanum lycopersicum	210	73.31/9.98	4	61	0.63	0.47

^aAssigned spot number corresponding to the number used in the respective figures

^b Identity of the proteins annotated by MALDI-TOF MS/MS

^cProtein database accession number (UniProt)

^dPlant species from which the protein was annotated

^eMASCOT score

^fTheoretical molecular mass and isoelectric point computed from ExPASy Mr/pI calculation tool

^gNumber of matched peptides of MSMS data with the corresponding protein in MSDB, SwissProt and NCBInr databases

^hPercentage of peptide sequences coverage for the identified protein by MS/MS

ⁱRegulation: The fold increase or decrese in % spot volume of each spot after the R. solanacearum inoculation. Variation: The ratio in the abundance of spot % spot volume between the species

^jThe results of SignalP (SiP) and SecretomP (SeP) analysis. Y presence of signal peptide evaluated from SignalP. The numbers are Secretom NN score calculated from SecretomP, in case no signal peptides were identified, and the NN score >0.5 was considered as secretory protein as suggested by the author

* Identity of the proteins revealed by LC-ESI-Ion Trap MS/MS