Table 3.

List of differential proteins in between healthy tomato plants of genotypes Hawaii7996 (resistant) and WVa700 (susceptible). The listed proteins were those consistently reproduced in three biological replications and are statistically significant (Student’s t test, P ≤ 0.05)

Spota	Identityb	Accessionc	Organismd	Scoree	Mr/pIf	Peptidesg	Coverageh	Variationi	SiP-SePj
1	Luminal-binding protein	P49118	Solanum lycopersicum	260	73.23/5.10	3	6	0.53	Y
2*	Enolase	P26300	Solanum lycopersicum	92	47.79/5.68	3	6	0.60	0.52
3	α-galactosidase, putative	Q9FWV8	Oryza sativa	137	44.66/5.47	2	5.6	1.79	Y
4	Peroxidase	Q9LWA2	Solanum lycopersicum	144	34.94/4.56	2	5.5	2.92	Y
5*	Hypothetical protein T8G24.2	Q9C6U3	Arabidopsis thaliana	88	34.70/7.17	5	3	1.69	0.61
6	Ferredoxin-NADP reductase	O04397	Nicotiana tabacum	92	41.95/8.67	2	5.9	1.38	0.73
7	Hypothetical protein	O04428	Citrus paradisi	92.8	32.64/5.46	1	4.1	0.38	0.50
8	Fructokinase-2	Q42896	Solanum lycopersicum	208	34.76/5.76	3	11	0.36	0.68
9*	Enolase	P26300	Solanum lycopersicum	291	47.79/5.68	5	14	0.50	0.52
10*	Oxygen-evolving enhancer protein 1	P23322	Solanum lycopersicum	423	34.98/5.91	12	34	2.00	0.45
11	NAC-alpha-like protein 3	Q6ICZ8	Arabidopsis thaliana	151	22.10/4.41	2	16	0.44	0.6
12*	Enolase	P26300	Solanum lycopersicum	181	47.79/5.68	4	15	0.41	0.52
13	Oxygen-evolving complex protein 2	P29795	Solanum lycopersicum	362	27.79/8.27	5	27	0.69	0.8
14	Translation initiation factor 5A-1	Q9AXQ6	Solanum lycopersicum	139	17.30/5.71	2	18	4.12	0.23

^aAssigned spot number corresponding to the number used in the respective figures

^b Identity of the proteins annotated by MALDI-TOF MS/MS

^cProtein database accession number (UniProt)

^dPlant species from which the protein was annotated

^eMASCOT score

^fTheoretical molecular mass and isoelectric point computed from ExPASy Mr/pI calculation tool

^gNumber of matched peptides of MSMS data with the corresponding protein in MSDB, SwissProt and NCBInr databases

^hPercentage of peptide sequences coverage for the identified protein by MS/MS

ⁱRegulation: The fold increase or decrese in % spot volume of each spot after the R. solanacearum inoculation. Variation: The ratio in the abundance of spot % spot volume between the species

^jThe results of SignalP (SiP) and SecretomP (SeP) analysis. Y presence of signal peptide evaluated from SignalP. The numbers are Secretom NN score calculated from SecretomP, in case no signal peptides were identified, and the NN score >0.5 was considered as secretory protein as suggested by the author

* Identity of the proteins revealed by LC-ESI-Ion Trap MS/MS