Table 4.
List of major basic proteins that were poorly resolved in 2-D IEF/SDS–PAGE but well separated in 3rd dimension SDS–PAGE
| Spota | Identityb | Accessionc | Organismd | Scoree | Mr/pIf | Peptidesg MS/MS |
Coverageh MS/MS |
SiP-SePi |
|---|---|---|---|---|---|---|---|---|
| Hawaii7996 (resistant genotype) | ||||||||
| 1 | Peroxidase | Q94IQ1 | Nicotiana tabacum | 480 | 39.06/5.99 | 8 | 27 | Y |
| 2 | Peroxidase prx14 | Q9M4Z3 | Spinacia oleracea | 139 | 37.22/9.29 | 2 | 7.7 | Y |
| 3* | Peroxidase | Q07446 | Solanum lycopersicum | 299 | 35.99/7.52 | 5 | 17 | Y |
| 3* | Pectinesterase | Q43143 | Solanum lycopersicum | 280 | 64.10/8.97 | 6 | 9 | Y |
| 4 | Glucan endo-1,3- β-glucosidase B | Q01413 | Solanum lycopersicum | 244 | 39.71/7.84 | 5 | 17 | Y |
| 5 | Osmotin-like protein precursor | Q41350 | Solanum lycopersicum | 404 | 27.26/8.15 | 6 | 29 | Y |
| WVa700 (susceptible genotype) | ||||||||
| 1* | Peroxidase | Q94IQ1 | Nicotiana tabacum | 391 | 39.06/5.99 | 6 | 14 | Y |
| 2 | Glucan endo-1,3-β-glucosidase B | Q01413 | Solanum lycopersicum | 172 | 39.71/7.84 | 2 | 6.7 | Y |
| 3 | Osmotin-like protein | Q41350 | Solanum lycopersicum | 284 | 27.26/8.15 | 2 | 10 | Y |
| 4 | Photosystem I reaction center subunit IV B | Q41229 | Nicotiana sylvestris | 131 | 15.22/9.74 | 2 | 19 | 0.82 |
| 5 | Oxygen-evolving complex protein 3 | Q672Q6 | Solanum lycopersicum | 201 | 24.57/9.64 | 3 | 11 | 0.65 |
| 6 | Oxygen-evolving complex protein 3 | Q672Q6 | Solanum lycopersicum | 73 | 24.57/9.64 | 1 | 4.3 | 0.65 |
| 7 | Photosystem I reaction center subunit II | P12372 | Solanum lycopersicum | 125 | 22.91/9.71 | 3 | 12 | 0.80 |
aAssigned spot number corresponding to the number used in the respective figures
b Identity of the proteins annotated by MALDI-TOF MS/MS
cProtein database accession number (UniProt)
dPlant species from which the protein was annotated
eMASCOT score
fTheoretical molecular mass and isoelectric point computed from ExPASy Mr/pI calculation tool
gNumber of matched peptides of MSMS data with the corresponding protein in MSDB, SwissProt and NCBInr databases
hPercentage of peptide sequences coverage for the identified protein by MS/MS
iThe results of SignalP (SiP) and SecretomP (SeP) analysis. Y presence of signal peptide evaluated from SignalP. The numbers are Secretom NN score calculated from SecretomP, in case no signal peptides were identified, and the NN score >0.5 was considered as secretory protein as suggested by the author
* Identity of the proteins revealed by LC-ESI-Ion Trap MS/MS