Table 2. Viability of 8-amino-dG containing M13 in E.colia.
Experiment no.b | Relative survival | |||
|
Control (%) |
|
8-amino-dG (%) |
|
|
–SOS |
+SOSc |
–SOS |
+SOSc |
1 |
100 |
100 |
71 |
93 |
2 |
100 |
100 |
135 |
130 |
3 |
100 |
100 |
93 |
72 |
Average | 100 | 100 | 100 | 98 |
aViability (%) was determined by comparing the number of plaques from the 8-amino-dG containing DNA with that of the control genome (assumed to have 100% viability).
bEach experiment refers to a separate construction of the control and the adducted genomes.
cSOS was induced by UV irradiation at 20 J/m2 as described by Bacolod et al. (24).