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. 2000 Nov;5(5):415–424. doi: 10.1379/1466-1268(2000)005<0415:mdithh>2.0.co;2

Fig 4.

Fig 4.

Melting profiles of HSP70B′ amplicons 1 to 4. Amplicons are designed to overlap and span the complete coding region of the gene. Graphs of the degree of naturation as a function of base position are shown for each of the amplicons. A 3°C temperature range in which mutations are predicted to be detected is shown for each amplicon. Melting profiles were calculated using the WAVEMAKERTM software