Table 1.

Origin of the seven nanovirus-positive faba bean samples from Morocco and their TAS-ELISA reactions with broad-spectrum and discriminating monoclonal antibodies (MAbs)

Faba bean sample	Collection site	Broad-spectrum MAbs to nanovirusesa	MAbs raised against FBNYV-Eg [13]				MAbs specific to FBNSV-Eth [17]
			1-3D8	2-5H9	2-3E12-D5	3-4A5	8-2G10	8-3G11	8-4F9	8-6F8-A5
Mor1	Sidi El Aidi, Settat, Chaouia	+++b	+++	−	−	+++	−	+	−	+
Mor4	Ouled Said, Settat, Chaouia	+++	+++	+	−	+++	−	++	−	++
Mor4-2	Ouled Abbou, Settat, Chaouia	++	++	−	−	++	−	−	−	−
Mor5	Boulaouane, El Jadida, Doukkala	+++	−	+	−	−	+++	+++	+++	+++
Mor19	Souk Sebt, Ben-Mellal, Tadla	+++	+++	+	−	+++	−	++	−	+
Mor23	Fkih Ben Salah, Ben-Mellal, Tadla	+++	+++	−	−	++	−	+	−	−
Mor23-2	Fkih Ben Salah, Ben-Mellal, Tadla	+++	+++	−	−	+++	−	++	−	+
FBNYV-Egc		+++	+++	+++	+++	+++	−	−	−	−
FBNSV-Ethc		+++	−	−	−	−	+++	+++	+++	++

^aBroad-spectrum MAbs used for nanovirus-specific detection were MAbs 1-1F2, 2-1A1, and 3-4F2 [13]

^bExtinction values (A_{405 nm}) for tenfold diluted leaf extracts and following a substrate incubation period of 1 h are shown. A_{405 nm} values were classed as +++ (>0.8), ++ (0.2–0.8), + (<0.2), and − (less than two times the A_405nm value for the non-infected control)

^cFBNYV-Eg and FBNSV-Eth, nanovirus isolates against which the MAbs had been raised, were used as control antigens