FIG. 2.

Inhibition of miR-1 represses SMC differentiation. (A, B) Regulation of SMC differentiation by miR-1 inhibitor. About 50 nM miR-1 inhibitor repressed expression of SMC-specific markers, including α-SMA and SMMHC, as evidenced by Western-blotting (C). ^*P < 0.05. (C) miR-1 inhibitor reduced the SMC differentiation efficiency determined by fluorescence-activated cell sorting. Left panel: Green histogram represents IgG2a isotype control and red represents anti-α-SMA of rat aorta SMCs (RASMCs) as positive control. Right panel: the proportion of the α-SMA-positive cells with green and blue representing inhibitor NC and miR-1 inhibitor, respectively. n = 4. PCR, polymerase chain reaction; NC, negative control; FITC, fluorescein isothiocyanate.