FIG. 3.

Impedance analysis, in real-time, of BG02 cells treated with increasing concentrations (0.02–20 μg/mL) of Hesca-2. Data were collected using an xCELLigence analyzer. About 2 × 10⁴ cells were seeded into each well of a microelectronic sensor array integrated-96-well plate. Antibody addition begins at 25 h and fresh media and antibody are added every 24 h thereafter. Arrows indicate time points when the plate was disconnected from the analyzer for cell feeding and to collect aliquots for LDH release analysis. These data are the average of quadruplicate treatments and standard deviations were <0.35, 0.20, 0.37, 1.02, and 1.40 for the 0, 0.02, 0.2, 10, and 20 μg/mL concentrations of Hesca-2, respectively. Similar but not identical trends were seen for the effect of Hesca-2 on cells of 1 × 10⁴ and 3 × 10⁴ seeding densities over the course of 72 h.