Table 1.

Preimplantation Development of siCdx2-Injected Embryos

Group	2-Cell stage formation/total (%)	Blastocyst formation (%)	Hatched blastocysts (%)
Not injected	34/34 (100)	29/34 (85.3)	17/26 (65.4)
siControl	259/261 (99.2)	227/261 (87.0)	43/71 (60.6)
siCdx2	248/250 (99.2)	221/250 (88.4)	0/86 (0)

Rates of cleavage, blastocyst formation, and hatching were recorded at 1.5, 3.5, and 6 days postcoitum, respectively. Fewer embryos were used for the hatching observation, as samples at different stages were randomly removed for other experiments. No significant differences were found until the blastocysts began hatching. All siCdx2-treated blastocysts failed to hatch and ultimately collapsed. Data are pooled from 3 independent microinjection experiments.