FIG. 1.

Flow cytometric analysis of CD14 cell surface molecule expression of epitope reactivity in freshly isolated peripheral blood leukocytes. Dot plot distribution of uncultured peripheral blood cells isolated using (A1) gradient density centrifugation or (A2) carbonyl iron incubation followed by gradient density centrifugation. The R1 gate corresponds to the size and granularity of neutrophils, R2 lymphocytes, and R3 monocytes. (R1–R3) Histogram analysis of mean fluorescence intensity of CD14 cell surface molecule expression in the gated areas. The shaded curves represent negative isotype control staining in each cell population. Open lines represent the mean fluorescence intensity for CD14. Neutrophils (R1) have low mean fluorescence intensity for CD14 expression, whereas monocytes (R3) have high mean fluorescence intensity. The small population of lymphocytes (R2) with low mean fluorescence intensity likely represents the activated B lymphocyte population (*).