FIG. 10.
Gene expression kinetics of CD14 in unsorted or CD14-positive selected equine bone marrow cells with or without LPS stimulation. MPCs were cultured 21 days and treated with LPS at dosages of 0, 1, 5, or 10 ng/mL media. (A) 18S gene amplification plot (to verify equal RNA loading). (B) CD14 amplification plot of LPS stimulated and control bone marrow cells. Control MPCs treated with 0 ng/mL of LPS (←) had higher Ct values (lower levels of CD14 expression) than cells that were treated with LPS at any dose (▸).