FIG. 11.

Flow cytometric analysis of cell surface molecule expression in sorted and unsorted bone marrow cells treated or not with trypsin. (A, B) Dot plot distribution of bone marrow cells cultured 30 days after separation at 2 days of culture into unsorted, positively, or negatively selected fractions using an antiequine CD14 antibody and MACS. Adherent cells were collected for analysis after 5 min of incubation at 37°C with either Accumax^® cell detachment solution (A) or 0.25% trypsin in Hanks balanced salt solution (B). (C–F) Histogram analysis of mean fluorescence intensity of cell surface molecule expression in Region 3. Shaded curves represent negative isotype control staining; open lines represent labeling for the cell surface markers indicated on the left-hand side. When trypsin was used to collect the cells for analysis, a decrease in mean fluorescence intensity was noted for some antibodies (F, *).