FIG. 12.

Flow cytometric analysis of cell surface molecule expression in bone marrow cells cultured for 30 days and treated with either Accumax or 0.25% trypsin in Hanks balanced salt solution. The percentage of positive cells using the respective antibody was compared between solutions (n = 4 ± SE). There were significant reductions (P ≤ 0.005) in the percentage of positive cells for some molecules (*) when trypsin was used instead of Accumax for cell preparation. The 0.25% trypsin solution reduces the detection of some, but not all cell surface markers in flow cytometric analysis.