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hMSCs increased secretion of TGFβ in cocultures. (Left panel) Human-specific ELISA of medium from cultures (t-test: **P < 0.01 vs. MSC). (Right panel) Western blot analysis of rNSCs cultured alone (NSC) or with hMSCs (coculture NSC) and hMSCs cultured alone (MSC) confirmed that hMSCs and rNSCs express both TGFβ receptors I and II. β-actin expression was used as a loading control. TGFβ, transforming growth factor β.
