FIG. 4.
Neural induction in mouse embryos lacking cilia and Ofd1 mutant missense EBs. (A) Expression of neural markers Nestin, Tuj1, and Ngn2 in E9.5 wild-type, Ift88, Kif3a, and Gli3 mutant embryos. (B) Sections of E9.5 wild-type and Ift88 mutant embryo neural tubes stained for postmitotic neurons (Tuj1, red), neural precursors (Nestin, green), and nuclei (DAPI, blue). Scale bar 20 μm. (C) Wholemount wild-type and Ofd1Gt EBs stained for Nkx2.2. Scale bar 20 μm. (D) Wild-type blastocysts stained for cilia (acetylated tubulin, green), basal bodies (Rootletin, red), and nuclei (DAPI, blue). Scale bar 10 μm. (E) qRT-PCR quantification of Sox1, Nestin, Sox3, and Tuj1 expression in Ofd1 missense mutant EBs. All expression changes between Ofd1 missense mutant and Ofd1Ofd1myc EBs are significant (p ≤ 0.05). Color images available online at www.liebertonline.com/scd