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A, B: Pulse-fix BrDU proliferation assay shows that the edge zone (EZ) of the blastoderm is virtually non-proliferative in comparison to the inner zone (IZ). The counter-stain is β-catenin (red). C, D: Pulse-incubate experiment shows that BrDU-positive nuclei are present in the EZ after 24 hr of additional incubation time following an initial BrDU pulse, which suggests that cells translocate from the IZ to the EZ during epiboly. Scale bar = 100 μm.
