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. 2010 Nov 8;20(5):795–807. doi: 10.1089/scd.2010.0343

FIG. 3.

FIG. 3.

Expression of transcription factors in MnOFiPS cells and MnOFs. (A, B) Transgene-specific PCR primers permit determination of the relative expression levels between total, endogenous (endo), and retrovirally expressed (transgene) genes (OCT4, SOX2, KLF4, and c-MYC) of MnOiPS cells (passage 4) and MnOFs using reverse transcription–real-time quantitative PCR. GAPDH is shown as a positive amplification and loading control. (C) Retrovirally expressed (transgene) genes are silenced in MnOFiPS cells clone 6 and 7 (Passage 18) indicated by reverse transcription–PCR. RNA from MnOFs is used as control and GAPDH is shown as a positive amplification and loading control. GFP carried by retroviral vectors is completely silenced in MnOFiPS cells (Passage 18). Bright field (BF). Scale bars, 100 μm. (D) Genomic DNA-amplified PCR products via transgene-specific primers. PCR, polymerase chain reaction.