Fig 5.

Binding of coat protein to GroEL. The increase in fluorescence of the WT and tsf binary complexes was followed at different ratios of coat protein to GroEL 14-mer. The corrected fluorescence intensity was plotted against the concentration of the protein being varied. (A) The concentration of coat protein was held constant at 0.2 μM while the concentration of GroEL was varied from 0 to 0.8 μM. (B) With the concentration of GroEL constant at 0.1 μM, the coat proteins were refolded at concentrations from 0 μM to 0.2 μM. The lines are drawn to aid the eye