Figure 3.

The expression of MIF mRNA in hepatocellular carcinoma (HCC) and healthy liver tissues. There were little changes in MIF gene expression in healthy liver tissues at the three time points. However, there was a twofold increase in MIF mRNA expression in HCC tissues at 24 h compared with healthy liver tissues (p < 0.05). The MIF mRNA levels in HCC tissues were keeping at a high level until 72 h compared with healthy liver tissues (p < 0.05). Semi-quantitative RT-PCR was performed in duplicate to minimize experimental error on the value calculated. All columnar values were expressed as means and standard deviations. A pattern of results was analyzed by repeating at least three times. p < 0.05 compared with the normal group.