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. 2000 Jul;5(3):229–242. doi: 10.1379/1466-1268(2000)005<0229:ptfhwt>2.0.co;2

Fig 4.

Fig 4.

Native TFIID complex in HeLa whole-cell extracts binds immobilized HSF1 C-terminal activation domains in vitro. (A) Western blot of transiently expressed TBP (T7-tagged) from whole-cell extracts bound to GST-AD2. (B) Western blot of endogenous TBP from HeLa whole-cell extracts bound to GST-AD2 and GST-AD1+2. Fifty microliters of whole-cell extract and 20 μL of GST-AD1+2 were used for the binding assays at the salt concentrations indicated. TBP was visualized by anti-T7 epitope monoclonal antibody or anti-hTBP. Lanes: C = GST negative control; Ip = input amount; 2 = AD2; 1+2 = AD1+2; M = marker consisting of T7-tagged TBP expressed in E. coli.