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Negative region of HSF1 binds TFIID in HeLa nuclear extracts. Nuclear extracts (500 μL) prepared from heat-stressed (HS+) and non–heat stressed (−) cells were used in binding reactions with immobilized GST and GST-negative region fusion proteins (GST-NR) in 100 mM KCl. The GST-NR construct contained the region from aa 212 to 310 of hHSF1. TBP was visualized by probing the blotted proteins with anti-TBP antibody
