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XAP2 is expressed in various established cell lines. Eight different cell lines were cultured, harvested, and homogenized. Total cytosolic extracts were isolated, and 75 μg of each lysate was resolved by SDS-PAGE, transferred to PVDF membrane, and analyzed by immunoblot analysis. XAP2 was detected with an XAP2 polyclonal antibody as primary antibody and [125I]-DAR as secondary antibody
