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Cellular XAP2 is unaffected by geldanamycin or radicicol treatment. Hepa-1 cells were treated with GA or radicicol at 100 nM and 2 μM for 6 h. Following treatment, cytosol was isolated, and 100 μg were resolved by SDS-PAGE and transferred to PVDF membrane, followed by immunoblot analysis with antibodies specific for the AhR, XAP2, and FKBP52. AhR and FKBP52 were visualized with [125I]-SAM and XAP2 with [125I]-DAR and subsequent autoradiography
