Fig 2.

Cell survival assay for acquired thermotolerance in EcB strain. Cells of WT, EcB, and ΔclpB1 grown at 37°C were shifted either directly to 54°C for 15 minutes (left column) or first preconditioned at 48.5°C for 1.5 hours before being exposed to 54°C for 15 minutes (right column). At the indicated time points, cells were removed and serially diluted from 1:1 to 1:10 000 and then spotted on BG-11 plates. Shown is a representative thermotolerance experiment of 3 independent replicas