Figure 4.

Histology comparison of AF tissue according to age and mature NP tissue. The cells were uniformly distributed in the newborn, and lacunae were observed surrounding them. (arrow in A) The newborn AF tissue was stained heavily with safranin-O, confirming that that the aged tissues are less in GAG than the newborn tissue (E, F, G). (circle in B) Newborn AF tissue shows numerous cells encapsulated with a material called lacunae; the mature AF tissue starts to show strains stacked in different diagonal directions with markedly reduced numbered cells and developed cell clusters (circle in B). In older AF tissue strains become blurred (C). NP tissue showing irregular direction and shape of strains. The NP cells have a rounder shape than the same age AF cells(D, H). (Note; the arrow indicated to cell; Newborn; 2 wks, 12 lb / mature; 6-7 mth, 150lb / older; 2-3 yr, 250-350 lb)