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. 2011 Apr 18;43(6):341–349. doi: 10.3858/emm.2011.43.6.037

Figure 6.

Figure 6

MLB attenuates NF-κB transcriptional activation and inflammatory chemokine MCP-1 production in HSCs. (A) HSCs were infected with the Ad5NF-κBLuc (MOI 500) for 12 h in DMEM containing 0.5% FBS. At 20 h post-infection, HSCs were treated with TNF-α (10 ng/ml) for 8 h with pretreatment of various concentrations of MLB (0-100 µM) for 1 h. Cells were lysed and NF-κB-mediated luciferase activity was quantified. Data represent the mean ± SD of 2 independent experiments and are expressed as fold-increase over unstimulated cells. All measurements of luciferase activity were normalized to the protein concentration. †P < 0.01 between no treatment and TNF-α only, ‡P < 0.01 compared with TNF-α alone. (B) Secreted MCP-1 in culture medium was quantified by ELISA. After 48 h serum starvation, HSCs were cultured in medium with 1% FBS for 24 h with various concentrations of MLB (0-100 µM). Data represent the mean ± SD of two independent experiments. *P < 0.05 compared with no MLB treatment, **P < 0.01 compared with no MLB treatment.