Table 2.
Summary of recombinant antigens used.
| Serovar D locus-tag (NC000117) | Name | Antigen details (kDa) | Serovar origin | DNA insert fragment size (bp) | Product size by SDS–PAGE (kDa) | Production of original expression clone | Fusion protein type | Recombinant expression and purification | Publications (cellular responses only) |
|---|---|---|---|---|---|---|---|---|---|
| CT812 | PmpD | Polymorphic outer membrane protein (polymorphic family of 9 genes A–I; 160) | L2 | 2.024 kb (690–2714 gene fragment) | 74 | Prof. Hill Gaston and Dr. Jane Goodall, University of Cambridge | His-tag | pPET/ProBond® | Goodall et al. (2001b) |
| CT681 | MOMP | Major outer membrane protein (40) | L1 | 1.6 kb (285–1182 gene fragment) | 33 | Prof. Ian Clarke, University of Southampton | B-gal | Induced insoluble inclusions purified by centrifugation resuspended in octylglucoside/DTT | Holland et al. (1993) |
| CT871 | PmpG | Polymorphic outer membrane protein (107) | A | 1.509 kb (127–1726 gene fragment) | 61 | Dr. Mark Felton, LSHTM | His-tag | pPET/ProBond® | Felton (2002) |
| CT587 | Enolase | Phosphopyruvate hydratase (enolase family; 45) | L2 | 1.274 kb (full length) | 45 | Prof. Hill Gaston and Dr. Jane Goodall, University of Cambridge | His-tag | pPET/ProBond® | Goodall et al. (2001b) |
| CT443 | Omp2 | Cysteine rich outer membrane protein (60) | L2 | 1.65 kb (700–2350 gene fragment) | 60 | Prof. Hill Gaston and Dr. Jane Goodall, University of Cambridge | His-tag | pPET/ProBond® | Goodall et al. (2001a) |