Table 2.
Specific activities of wild type and mutant SOR, iron content, numbers (#) of preparations and assays per preparation.
| Mutant | Oxygenase spec. activity (U/mg protein) | Reductase spec. activity (U/mg protein) | Fe content absolute (nmol/2.8 nmol protein) | Fe content relative (nmol/nmol subunit) | # Preps | # Assays |
|---|---|---|---|---|---|
| Wild type | 3.03 ± 0.31 | 1.69 ± 0.44 | 2.8 | 1 | 4 | 2 |
| TETRAMER CHANNEL MUTANTS | |||||
| delL | 12.74 | 12.02 | 3.0 | 1.1 | 3 | 3 |
| delK | 9.89 ± 1.48 | 8.05 ± 3.14 | 3.1 | 1.1 | 4 | 3 |
| F133A | 3.89 | 1.48 | nd* | nd* | 2 | 2 |
| F141A | 4.43 | 1.96 | 4.3 | 1.6 | 2 | 2 |
| F133A/F141A | 5.88 | 5.87 | 4.8 | 1.72 | 2 | 2 |
| ACTIVE SITE PORE MUTANTS | |||||
| M296V | 3.03 ± 0.31 | 2.74 ± 0.71 | 3.9 | 1.4 | 3 | 3 |
| M297A | 1.39 ± 0.27 | 0.74 ± 0.05 | 4.3 | 1.5 | 3 | 3 |
| MM296/297VT | 1.89 ± 0.11 | 0.94 ± 0.09 | 4.0 | 1.4 | 3 | 3 |
| MM296/297TT | 2.26 ± 0.57 | 0.98 ± 0.05 | 4.4 | 1.6 | 3 | 3 |
| TRIMER CHANNEL MUTANTS | |||||
| R99A | 5.53 ± 1.21 | 2.55 ± 0.57 | 4.0 | 1.4 | 4 | 3 |
| R99I | 4.25 ± 0.72 | 3.82 ± 1.07 | 2.9 | 1.0 | 1 | 3 |
| S226A | 4.12 ± 0.92 | 2.21 ± 0.47 | 3.4 | 1.2 | 4 | 3 |
| S226T | 5.54 ± 1.34 | 2.64 ± 0.26 | 3.4 | 1.2 | 4 | 3 |
| S226I | 3.1 ± 0.98 | 5.15 ± 0.68 | 3.1 | 1.1 | 1 | 3 |
| S226L | 2.68 ± 0.35 | 8.39 ± 1.19 | 2.9 | 1.0 | 1 | 3 |
| Zn BINDING SITE MUTANTS | |||||
| H277A | 2.73 ± 0.46 | 1.71 ± 0.76 | 3.3 | 1.2 | 2 | 2 |
| H166A | 2.9 ± 0.22 | 1.38 ± 0.21 | 6.9 | 2.4 | 2 | 2 |
*Not determined.