Skip to main content
. 2011 Apr 18;2:78. doi: 10.3389/fmicb.2011.00078

Figure 3.

Figure 3

Francisella novicida does not reside in acidic compartments within H. vermiformis cells. (A) Representative confocal microscopy images of colocalization of FCVs with the LysoTracker dye by the GFP-expressing wt F. novicida and iglC at 30 min, 1, and 12 h post-infection is shown. Uninfected cells were used as a negative control, while formalin killed bacteria served as a positive control. The images are representatives of 100 infected cells examined from three different cover slips. The results shown are representative of three independent experiments. (B) Quantification of colocalization of the LysoTracker DND-99 dye with the FCVs of the bacterium at 30 min, 1, and 12 h post-infection is shown. The results shown are representative of three independent experiments, and error bars represent standard deviations of triplicate samples.