Figure 2.

Predicted change in the aggregation propensity after mutation compared with in vivo experimental solubility in Escherichia coli cytosol for HypF-N variants. Each graph reports the predicted change in the aggregation propensity after mutation (calculated according to the algorithm indicated in each graph and following the procedure described in the supplementary information online) compared with experimental solubility in E. coli cytosol (I_SN/I_P) of the mutants of HypF-N, as described previously (Winkelmann et al, 2010). A value of 0 on the y-axis corresponds to a value of 0.5 on the x-axis (no change, relative to wild type). Scales on the y-axis have been adjusted to show, for each plot, the full data set. The lines represent the best fits of the data to linear functions. For each plot, the name of the algorithm used is reported, as well as the absolute value of the Pearson linear correlation coefficient (r) and the statistical significance of the slope (p).