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. 2011 May 3;2:96. doi: 10.3389/fmicb.2011.00096

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Copyright © 2011 Thywißen, Heinekamp, Dahse, Schmaler-Ripcke, Nietzsche, Zipfel and Brakhage.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Influence of A. fumigatus wild-type-derived melanin ghosts on phagolysosomal acidification. (A) Percentage of intracellular conidia residing in acidified phagolysosomes after ingestion by MH-S cells. Latex beads were used as a positive control. Values represent the mean + SD of three experiments. (B) Representative micrographs showing colocalization of melanin ghosts, stained prior to phagocytosis with biotinylated anti-melanin camelid antibody and Cy5.5 conjugated streptavidin (red), with acidified compartments visualized by LysoTracker labeling (green). Size bar, 2 μm.