Table 1.
Twenty-one human malignant melanoma cell lines and 3 normal control melanocytes
| Malignant melanoma cell lines | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| No. | Name* | Disease | Biopsy site | Xenograft animal models |
Age | Gender | Source and reference | This study |
|
| Tumorigenicity | Metastasis | Type | |||||||
| 1 | A2058 (5) (A) | MM | Lymph node | Yes | Yes | 43 | Male | ATCC (30) | A |
| 2 | A375 (5) (B) | MM | Skin | Yes | Yes | 54 | Female | ATCC (30, 31) | A |
| 3 | C32 (5) (B) | MM | Skin | Yes | Unknown | 53 | Male | ATCC | A |
| 4 | COLO829 (5) (E) | MM | Skin | Unknown | Unknown | 45 | Male | ATCC | A |
| 5 | G361 (5) (C) | MM | Skin | Yes | Yes | 31 | Male | ATCC (30) | A |
| 6 | HT144 (5) (C) | MM | Skin | Yes | Yes | 29 | Male | ATCC (32) | A |
| 7 | HTB65 (5) (B) | MM | Lymph node | Yes | Yes | 78 | Male | ATCC (31, 33) | A |
| 8 | RPMI7951 (5) (B) | MM | Lymph node | Yes | Unknown | 18 | Female | ATCC | A |
| 9 | SKMEL2 (5) (B) | MM | Skin of thigh | Yes | Unknown | 60 | Male | ATCC | A |
| 10 | SRS3 (9) (E) | UACC903(+6) variant | Yes | Unknown | (16) | A | |||
| 11 | UACC3074 (5) (E) | MM | Unknown | Unknown | Unknown | Unknown | Unknown | UACC | A |
| 12 | WM266-4 (5) (b) | MM | Skin | Yes | Yes | 58 | Female | ATCC (34) | A |
| 13 | C8161 (4) (E) | MM | Unknown | Yes | Yes | Unknown | Unknown | (31) | B |
| 14 | MUM2C (4) (E) | MM (Uveal) | Liver | Yes | Yes | Unknown | Male | (23) | B |
| 15 | UACC1227 (5) (F) | MM | Lymph node | Unknown | Unknown | 27 | Male | UACC | B |
| 16 | UACC2565 (5) (F) | MM | Lymph node | Unknown | Unknown | 63 | Male | UACC | B |
| 17 | UACC457 (5) (F) | MM | Abdomen | Unknown | Unknown | 41 | Male | UACC | B |
| 18 | UACC827 (5) (F) | MM | Right breast | Unknown | Unknown | 32 | Female | UACC | B |
| 19 | UACC903 (9) (E) | MM | Back | Yes | Unknown | 35 | Female | UACC (17) | B |
| 20 | UACC903(+6) (11) (E) | UACC903 variant | Yes | Unknown | (17, 35) | B | |||
| 21 | UACC929 (5) (F) | MM | Lymph node | Unknown | Unknown | 31 | Male | UACC | B |
| Normal control melanocytes | ||||||
|---|---|---|---|---|---|---|
| No. | Name | Description | Biopsy site | Age | Gender | Source and reference |
| 1 | HEMn-LP (9) (G) | Normal human epidermal melanocytes, lightly pigmented | Foreskin | Neonatal | Male | Cascade Biologics |
| 2 | HEMn-MP (9) (G) | Normal human epidermal melanocytes, moderately pigmented | Foreskin | Neonatal | Male | Cascade Biologics |
| 3 | HEMn-DP (9) (G) | Normal human epidermal melanocytes, darkly pigmented | Foreskin | Neonatal | Male | Cascade Biologics |
Abbreviation: MM, malignant melanoma.
*
All cell lines were cultured in this laboratory to 90% confluence for harvesting of cells at the passages (the numbers in parentheses) after receiving from the resources indicated and analysis. The letters in parentheses indicate culture medium used: (A) DMEM, (B) Eagle's MEM, (C) McCoy's 5A, (E) RPMI 1640, (F) L-15, and (G) Medium 254. The cell lines cultured in L-15 medium were under 100% air, that is, without the supplement of 5% carbon dioxide. All other 19 cell lines were cultured in the presence of 5% carbon dioxide.