Fig. 3. Tat-Bcl-xL and Tat-BH4 decreased apoptosis 7 days after injury.
Elisa assays were use to measure the levels of oligonucleosomes in the cytosol 7 days after SCI in cords isolated from four groups of rats: (a) sham-and vehicle treated (n=7); (b) SCI+vehicle (n=5); or (c) SCI+Tat-Bcl-xL (n=5); and (d) SCI+Tat-BH4 (n=5). In all measurements we used 1cm-long thoracic segments containing the site of injury, T10. Both Tat-Bcl-xL and Tat-BH4 treatment significantly decreased apoptosis compared to vehicle-treated animals. Data represent mean ± SD. * p<0.05 compared to sham-vehicle group; † p<0.05 compared to the injury-vehicle treated group; Two way ANOVA with Tukey’s post-hoc correction).