Table 1.
Description of experimental groups
| Time after injury |
Groups | Drug delivery |
n (ELISA, WB) |
n (H,IHC) |
n (BBB) |
Total |
|---|---|---|---|---|---|---|
| 24h | Sham + Vehicle | 1ul/hr for 24h | 4 | 3 | NA | 7 |
| Sham + Tat-Bcl-xL | 10ug/day | 4 | − | NA | 4 | |
| Injury + Vehicle | 5 | 3 | NA | 8 | ||
| Injury + Tat-Bcl-xL | 5 | 3 | NA | 8 | ||
| 7days | Sham + Vehicle | 0.5ul/hr for 7 | 7 | 3 | NA | 10 |
| Injury + Vehicle | days | 7 | 3 | NA | 10 | |
| Injury + Tat-Bcl-xL | 5ug/day. | 7 | 3 | NA | 10 | |
| Injury + Tat-BH4 | 7 | 3 | NA | 10 | ||
| 60 days | Sham + Vehicle | 0.5ul/hr for 7 | 3* | 3 | 10 | 10 |
| Injury + Vehicle | days | 3* | 3 | 10 | 10 | |
| Injury + Tat-Bcl-xL | 5ug/day. | 3* | 3 | 8# | 10 | |
| Injury + Tat-BH4 | 3* | 3 | 10 | 10 | ||
| TOTAL | 48 | 105 | ||||
*
Only WB analysis but no Cell death ELISA was performed in this group.
#
An additional injury at the site of catheter implantation was observed at the time of perfusion in two Tat-Bcl-xL treated animals. Data from these animals were omitted from the analysis. n(ELISA, WB), number of animals for which apoptotic cell death was measured by ELISA and analysis of Bcl-xL levels were quantified by western blot. n(H, IHC), number of animals used for histology and immunohistochemistry analysis. n (BBB), number of animals for which BBB scores were obtained. NA, Not applicable