Figure 3.

Restriction endonuclease assay to detect editing of the transgene transcripts. (A) Sau3AI (S) restriction map of the edited or non-edited RT–PCR products of the MR413 transgene with primers Trps16α3.1 and PPrrn2 (130 bp product). The size in bp is written under each restriction fragment. (B) NuSieve agarose gel (4%) of a Sau3AI restriction digest of 130 bp PCR product. PCR was performed on cloned and sequenced, edited (MR413T) or non-edited (MR413C) cDNA. Lanes 1–6 represent independent cloned RT–PCR products from a MR413 plant. Lane 5 corresponds to an edited RT–PCR product.