Table 1. Analysis of rpoB site II editing in transplastomic plants.
| Construct | Transgene | Endogenous | Nucleotides | ||||
| |
edited |
edited |
–20 |
–7 |
+2 |
||
| MR214 | 7/16 | + | 27/28 | + | A | G | G |
| MR215 | 1/75 | – | 22/22 | + | T | A | A |
| MR411 | 17/96 | + | 37/42 | + | A | G | G |
| MR412 | 0/69 | – | 17/18 | + | T | G | G |
| MR413 | 29/91 | + | 22/24 | + | A | A | G |
| MR414 | 13/45 | + | 20/21 | + | A | G | A |
| MR415 | 2/45 | – | 25/28 | + | T | A | G |
| MR416 | 0/32 | – | 27/28 | + | T | G | A |
| MR417 | 9/35 | + | 22/23 | + | A | A | A |
| MR419 | 2/45 | – | 25/26 | + | T | A | A |
The number of edited rpoB site II transcripts is shown for both the transgene and the endogenous tobacco rpoB sequences. The nucleotide columns identify differences between tobacco (MR211, MR411) and black pine (MR215) rpoB sequences in the region –20 to +6; bold underlined nucleotides are those encoded in black pine. Editing status was derived both by sequencing of individual cDNA clones and by the restriction digestion assay (see Materials and Methods).