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. 2011 Apr;12(2):123–129. doi: 10.2174/138920211795564340

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©2011 Bentham Science Publishers Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. (1). — recombinant yeasts expressing Trametes sp. C30 lacase genes grown on selective medium (SGal URA). From top to bottom and left to right: URA3 based pYES-DEST52 constructs containing each of the five cDNAs lac1, lac 2, lac 3, lac 4, lac 5 inserted under the control of the regulated GAL1 promoter. The halo around colonies results from the oxidation of the 2-methoxyphenol of the medium by secreted active laccases.