Fig 5.

 The amount of Hsp72 increases to a similar extent in adult and aged rat skeletal muscles after heat shock. Proteins from muscles were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred to nitrocellulose, and reacted with a Hsp72-specific antibody as described in the text. Lane 1: unstressed (control) aged rat skeletal muscle; lane 2: heat shocked aged rat skeletal muscle; lane 3: unstressed (control) adult rat skeletal muscle; lane 4: heat shocked adult rat skeletal muscle. (A) Soleus muscle. (B) White gastrocnemius muscle