Steps possible on a low order
complex
|
Steps requiring a higher order complex
|
| Individual RSS binding |
Enforcement of the 12/23
rule |
| Nicking |
Coordinated cleavage at two RSSs |
| Hairpin formation |
Potential isomerization of the
four-end complex, to direct pairing of ends for joining |
| Retention of coding
DNA after cleavage |
Potential coordination of coding
end joining‚ with signal end joining |
| Hairpin opening/processing |
Prevention of translocation |
| Recruitment of repair
proteins |
|
| Obscuring of cut ends from damage
sensors |
|
