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. 2011 May 13;286(27):23725–23734. doi: 10.1074/jbc.M110.213868

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — MdmX ubiquitination by Mdm2 and effects of p53 on Mdm2 and MdmX ubiquitination. A, effects of p53 on Mdm2 autoubiquitination. In vitro Mdm2 autoubiquitination was analyzed in the presence of different concentrations of MdmX (0–900 nm) and the presence (+p53) or absence of p53 (−p53). Ubiquitinated Mdm2 was detected by Western blotting of HA (recombinant Mdm2 is HA-tagged) and was indicated by Ub-Mdm2. SG, stacking gel. B, effects of p53 on Mdm2-mediated MdmX ubiquitination. In vitro MdmX ubiquitination was analyzed in the presence of different concentrations of Mdm2 (0–900 nm) and the presence (+p53) or absence of p53 (−p53). Ubiquitinated MdmX was detected by Western blotting of FLAG (recombinant MdmX is FLAG-tagged).