TABLE 1.

Kinetic and thermodynamic parameters of the interaction between LLT1 and CD161 at 25 °C

Immobilized	Analyte	Kineticsa
		Kd, eqb	kon (× 105)	koff	Kd, kinc
		μm	m−1s−1	s−1	μm
CD161	Wild type LLT1	52.6
	C163S LLT1	51.8
	H176C LLT1	48.2	1.1 ± 0.1	5.3 ± 0.55	48.5 ± 7.5

		Thermodynamicsd
		ΔG	ΔH	−TΔS	ΔCp
		kcal/mol		kcal/mol/K
CD161	H176C LLT1	−5.9 ± 0.02	−3.2 ± 0.13	−2.7 ± 0.11	−0.41 ± 0.01

^a The values are means ± range of two experiments.

^b K_d, eq values were obtained from the equilibrium analysis.

^c K_d, kin values were obtained from global fitting.

^d The values are means ± S.E. of three experiments.