TABLE 2.
Binding affinities of LLT1 mutants for immobilized CD161 mutants at 25 °C
| CD161 |
Kd |
||||||
|---|---|---|---|---|---|---|---|
| WT LLT1 | Y165A | N167A | K169E | R175E | R180E | K181E | |
| μm | |||||||
| Wild type | 48 | 240 | 180 | NBa | NB | NB | NB |
| E162R | 310 | NB | NB | NB | NB | NB | NB |
| E179R | 34 | 150 | 120 | 300 | NB | NB | NB |
| E179A | 393 | NB | NB | NB | NB | NB | NB |
| R181E | 100 | 210 | 140 | NB | NB | NB | NB |
| D183R | 390 | NB | NB | NB | NB | NB | NB |
| K185E | 55 | 170 | 140 | 310 | NB | NB | NB |
| K185A | 236 | 180 | 149 | 318 | NB | NB | NB |
| E186R | 150 | NB | NB | NB | NB | NB | NB |
| Y198A | NB | NB | NB | NB | NB | NB | NB |
| E200R | 45 | NB | NB | NB | NB | NB | NB |
| E200A | NB | NB | NB | NB | NB | NB | NB |
| Y201A | NB | NB | NB | NB | NB | NB | NB |
| E205R | 370 | NB | NB | 26 | NB | NB | NB |
a NB, no detectable binding.