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. 2011 Apr 18;286(27):23877–23887. doi: 10.1074/jbc.M111.234682

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FIGURE 7. — Transient kinetic analysis of UDP-Glc oxidation by wild-type hUGDH. A, stopped-flow progress curves for reactions at varied NAD⁺ concentration in the range 0.25–10 mm. The concentration of UDP-Glc was 1 mm. Gray lines show data and black lines are fits with Equation 1. The pre-steady-state burst is indicated by extrapolation. E is the concentration of hUGDH subunits. B, dependence of the pre-steady-state burst on the concentration of NAD⁺ used. C, formation of NADH and release of proton during oxidation of UDP-Glc (1.0 mm) by NAD⁺ (0.1 mm). Black lines are fits of data with Equation 1.