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. 2011 May 12;286(27):23888–23899. doi: 10.1074/jbc.M111.245969

FIGURE 5.

FIGURE 5.

Lys-C cleavage of secretin receptor labeled by each of position 15, 20, and 25 probes. Top, diagram of the sites of Lys-C cleavage of the first CNBr fragment (Ala1–Met51) of the secretin receptor along with the masses of the protein cores of the resultant fragments. Bottom, Lys-C cleavage of the CNBr fragment of the secretin receptor labeled by each probe resulted in a non-glycosylated band migrating at approximately Mr = 6,000, representing the labeling of the Ala1–Lys30 fragment at the distal amino terminus of the receptor. EF, endoglycosidase F.