FIGURE 6.
CNBr cleavage of V16M secretin receptor labeled by position 15, 20, and 25 probes. Top, diagrams of the sites of CNBr cleavage of the Ala1–Met51 region of the V16M mutant secretin receptor labeled by each probe. Bottom, CNBr cleavage of the V16M mutant receptor labeled by the position 20 probe yielded a non-glycosylated band migrating at approximately Mr = 6,000, representing the labeling of the amino-terminal Ala1–Met16 fragment. CNBr cleavage of the V16M mutant receptor labeled by position 15 and 25 probes each yielded a fragment migrating at approximately Mr = 17,000 that shifted to approximately Mr = 8,000 after deglycosylation, representing the labeling of the carboxyl-terminal Leu17–Met51 fragment. EF, endoglycosidase F.