FIGURE 8.

Identification of labeled receptor residues by radiochemical sequencing. Shown are radioactive elution profiles of Edman degradation sequencing of the purified receptor fragments labeled by each of the position 2 (Ile¹⁹⁸–Met²⁰⁵), 15 (Leu¹⁷–Met⁵¹), 20 (Ala¹–Met⁵¹), 24 (Pro⁹⁷–Lys¹¹⁹), and 25 (Leu¹⁷–Met⁵¹) probes. There were peaks in eluted radioactivity in cycles 2, 3, 2, 1, and 7 that correspond with covalent attachment to receptor residues Leu¹⁹⁹, Glu¹⁹, His², Pro⁹⁷, and His²³ by position 2, 15, 20, 24, and 25 probes, respectively.