FIGURE 2.

Adenovirus-mediated VP16-LXRα and VP16-LXRβ overexpression accelerate mineralization and triglyceride accumulation, whereas LXRαDN and LXRβDN ameliorate these effects in CVCs under high phosphate. CVCs were treated with adenoviruses at an MOI of either 20 or 40. After 12 h of adenovirus treatment, the medium was replaced with DMEM containing 15% FBS and 2.0 mm phosphate for 10 days. A, Alizarin Red staining. B, calcium content. C, TG content. D, ALP and COL1A1 mRNA. **, p < 0.001 versus Ad-empty with 2.0 mm phosphate. Data represent the mean ± S.E.