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. 2011 May 14;286(27):23982–23995. doi: 10.1074/jbc.M111.220491

FIGURE 1.

FIGURE 1.

Association between PPARγ and chemerin during adipogenesis of bone marrow-derived mesenchymal stem cells. Differentiation of BMSCs into mature adipocytes (day 14) was associated with the intracellular accumulation of lipid droplets (A) and an induction of chemerin and PPARγ gene expression (B). Chemerin and PPARγ mRNA levels were measured using qPCR and expressed relative to undifferentiated BMSCs (day 0). Immunoblots of BMSCs during adipogenesis showed an induction of chemerin (C) and PPARγ (D) protein expression in a differentiation-dependent manner. Knockdown of either chemerin (CHE-KD) or PPARγ (PPARγ-KD) gene expression using adenovirally delivered shRNAs markedly reduced adipogenesis as assessed by the quantification of oil red O staining (E) and expression of chemerin and PPARγ (F). Error bars represent S.E. (n = 3). *, p < 0.05 versus day 0 (B); *, p < 0.05 versus the no virus and LacZ knockdown (LZ-KD) controls (E and F).