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. 2011 May 20;286(27):24007–24014. doi: 10.1074/jbc.M111.223289

FIGURE 7.

FIGURE 7.

Dimerization of the Flv1 and Flv3 proteins in the WT and mutant cells. Soluble fraction of cellular proteins was isolated as described (6). Protein complexes were separated using 6–13% gradient blue native PAGE electrophoresis (A) and immunodetected with Flv3 antibody (B). The arrow indicates the location of the Flv3 protein where it is present as a dimer with molecular mass of ∼140 kDa. The molecular mass of the Flv monomer is 70 kDa. Gels were loaded on a protein basis.