Skip to main content
. 2011 May 16;286(27):24089–24100. doi: 10.1074/jbc.M111.219733

FIGURE 7.

FIGURE 7.

Effect of AR inhibition on hypoxia-mediated expression of structural proteins and AR-catalyzed reaction products and hypoxia-mediated HIF-1α expression in HT29 cells. A and C, the growth-arrested HT29 cells were treated with hypoxia and/or FGF, serum, HNE, GS-HNE esters, or GS-DHN esters for 24 h, and Western blot analysis was performed in total cell lysates using antibodies against LOX2, uPAR, MT1-MMP, MMP2, c-MET, vimentin, HIF-1α, and GAPDH. Fold values of normalized intensity measured by densitometric scanning. B, uPAR overexpressing HT29 cells were incubated with fidarestat (Fid), or the carrier was subsequently stimulated with hypoxia and/or FGF for another 24 h, and cell viability was measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Insets show the Western blot analysis for uPAR protein in untransfected, control plasmid (Cont-Plasmid), and uPAR overexpressing plasmid transfected cell extracts. Bars represent mean ± S.E. (n = 4). *, p < 0.001 compared with control (Con) and #, p < 0.001 compared with cells treated with hypoxia and/or FGF or serum. C, control; CP, control plasmid; UP, uPAR plasmid.